• Salinomycin Workflows for HCC Research

  2026-08-22

  Build more informative hepatocellular carcinoma assays with Salinomycin by pairing proliferation, apoptosis, calcium, transporter, and Wnt/β-catenin readouts. This workflow-oriented guide helps distinguish cytostatic effects from cell death while addressing ionophore-specific formulation, exposure, and toxicity considerations.

• Gemcitabine: Mechanism and Research Workflows

  2026-08-22

  Gemcitabine is a DNA synthesis inhibitor that creates replication stress and supports mechanistic cancer research. Its strongest practical use is in integrated apoptosis assay and DNA damage response assay workflows, while concentration and response remain model dependent.

• Gramine, CUL3–MTDH, and Ferroptosis in TNBC

  2026-08-21

  A 2026 study identifies gramine as a potential triple-negative breast cancer therapy that activates ferroptosis through the CUL3–MTDH ubiquitination axis. Its combination of target-engagement assays, mechanistic rescue experiments, and xenograft validation provides a useful framework for evaluating ferroptosis-inducing compounds while highlighting the need for orthogonal viability and mechanism-specific measurements.

• Cl-Amidine Workflows for PAD4 Research

  2026-08-20

  Build cleaner PAD4 inhibition experiments with practical guidance for biochemical assays, immune-cell studies, and disease-model validation. This workflow positions Cl-Amidine as a mechanistic tool while clearly separating established PAD4 applications from exploratory links to mitochondrial and lung-injury biology.

• Palbociclib: A Cell-Cycle Lens on DNA Repair

  2026-08-20

  Palbociclib (PD0332991) is more than a CDK4/6 inhibitor: it can serve as a mechanistic probe for separating cell-cycle state from DNA-repair phenotypes. This guide connects its RB-dependent activity with ERCC1- and p53-informed assay design in cancer biology research.

• Thapsigargin: From Calcium Flux to Translation

  2026-08-19

  Thapsigargin is more than a laboratory stressor: as a SERCA pump inhibitor, it provides a controllable bridge between intracellular calcium dynamics, endoplasmic reticulum stress, inflammasome signaling, and apoptosis. This thought-leadership guide shows translational researchers how to use that bridge to design stronger assays and interpret preclinical findings without overstating therapeutic relevance.

• Rifampin for Transcription Control Research

  2026-08-19

  Rifampin is a rifamycin antibiotic that provides a precise bacterial transcriptional perturbation for resistance, regulation, and synthetic biology research. This guide connects its assay value to a light-inducible translational gene switch while clarifying what the comparison can—and cannot—demonstrate.

• Hot-Melt Extrusion Improves Ziprasidone Bioavailability

  2026-08-18

  This AAPS PharmSciTech study developed a Plasdone-S630/HPMCAS-HF solid dispersion of ziprasidone hydrochloride using hot-melt extrusion and optimized it with central composite design. The formulation increased exposure in fasted beagle dogs while eliminating the pronounced food-dependent absorption observed with the marketed reference product, providing a practical strategy for improving oral delivery of a poorly soluble drug.

• DiscoveryProbe Bioactive Compound Library Plus Workflow

  2026-08-18

  The DiscoveryProbe Bioactive Compound Library Plus connects broad chemical profiling with focused thermal-shift, biochemical, and cellular follow-up. Its pre-dissolved format supports practical screening across bacterial sensor proteins, apoptosis assays, cancer research, and pathway-focused drug discovery.

• SN-38 Disrupts FUBP1–FUSE DNA Binding

  2026-08-17

  The reference study identifies an additional mechanism for camptothecin and SN-38: inhibition of the FUBP1 interaction with its single-stranded DNA target, FUSE. By combining an FDA-approved drug-library screen, AlphaScreen binding assays, and cellular analysis in hepatocellular carcinoma models, the work expands interpretation of SN-38 beyond conventional topoisomerase I inhibition.

• TEAD2, Ferroptosis, and HCC: Insights from Ren et al.

  2026-08-17

  Ren et al. integrated public cancer databases with cellular validation to identify TEAD2 and TEAD4 as overexpressed in hepatocellular carcinoma, with TEAD2 linked to adverse survival. Their findings further position TEAD2 as a potential regulator of ferroptotic vulnerability through iron accumulation and oxidative damage, while highlighting associations between TEAD expression and tumor immune infiltration.

• V5 Epitope Tag Peptide: From Assay to Translation

  2026-08-16

  A mechanistic and strategic guide to using the V5 tag for reproducible protein detection, antibody validation, multiplex imaging, and translational assay design.

• HyperPFU™ high-fidelity DNA polymerase: PCR Guide

  2026-08-15

  HyperPFU™ high-fidelity DNA polymerase is intended for accurate amplification of long, GC-rich, inhibitor-affected, or otherwise difficult DNA templates. It is appropriate for blunt-ended products used in cloning and sequencing, but not for workflows that require 3′-A overhangs or polymerase-generated sticky ends.

• CAY10499: Inhibitor of Human Hormone Sensitive Lipase

  2026-08-14

  CAY10499 combines nanomolar human HSL inhibition with measurable MGL and FAAH activity, making it useful for separating lipase-driven lipid flux from ACLY-dependent macrophage reprogramming. This workflow-oriented guide covers enzyme assays, monocyte-to-macrophage models, EV studies, controls, and troubleshooting.

• AR Heterogeneity Shapes Enzalutamide Response

  2026-08-14

  The reference study shows that androgen receptor expression patterns define biologically distinct castration-resistant prostate cancer cell states with different responses to castration and Enzalutamide. By combining patient-tissue profiling, genome-edited LNCaP models, xenografts, transcriptomics, and combination treatment experiments, the authors identify AR-low disease and BCL-2 dependence as important considerations for resistance research.

8649 records 1/577 page Next 12345 下5页 Last page