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An important concern regarding stem cell based therapies is
2018-11-08
An important concern regarding stem cell based therapies is the potential for unwanted cell growth. In a previous study we showed that the TheraCyte encapsulation device is durable enough to withstand freezing without evidence of cell escape, suggesting that once buy Lomefloxacin HCl are sealed in
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One attractive strategy to improve outcome in heart failure
2018-11-08
One attractive strategy to improve outcome in heart failure is to regenerate damaged myocardium by making more functional cardiomyocytes. Extensive work over the past two decades has overturned the prevailing dogma that adult cardiomyocytes do not undergo cell division by demonstrating that adult ca
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br Materials and methods br Results br
2018-11-08
Materials and methods Results Discussion riPSCs are typically generated and cultured on MEF feeders (Liu et al., 2008). Feeders are sufficient to support rhesus iPSC long-term maintenance. However, this approach is too labor intensive for large-scale culture. Optimization of this culture sy
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In the present study we tested five different pharmacologica
2018-11-08
In the present study, we tested five different pharmacological agents that are commonly used as selective inhibitors of Nodal/Activin signaling, namely SB431542, LY364947, A-83-01, SB505124, and SB525334 (Inman et al., 2002; Callahan et al., 2002; Laping et al., 2002; DaCosta Byfield et al., 2004; L
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br Conclusion Human BMSCs are a promising cell
2018-11-08
Conclusion Human BMSCs are a promising cell type for regenerative medicine and tissue-engineering applications. They have the capacity for self-renewal and exhibit multipotent differentiation potential through which they can produce lineages such as osteoblasts, chondrocytes, and adipocytes (Char
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Human embryonic stem cells hESC
2018-11-08
Human embryonic stem silybin (hESC) provide an accessible platform for modeling cardiovascular differentiation in vitro, thus permitting the interrogation of signaling pathways that are specifically active in embryonic endothelium. Moreover, hESC-derived ECs can recapitulate fundamental cellular ev
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br Materials and methods br Acknowledgments This
2018-11-08
Materials and methods Acknowledgments This work was supported by grants from the National Basic Research Program of China (973 program 2011CB964901 and 2012CB944901), the National Natural Science Foundation of China (81101510 and 31101053), Natural Science Foundation of Hunan Province (14JJ200
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Introduction Multipotent stromal cells also referred
2018-11-08
Introduction Multipotent stromal cells, also referred to as mesenchymal stem glp-2 cost (MSCs), are an heterogeneous subset of stromal cells present in several tissues including bone marrow, bone, adipose tissue, skin, kidney, umbilical cord and placenta (Friedenstein et al., 1968). The minimal cr
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br Conclusions All approaches described
2018-11-08
Conclusions All approaches described in this review for in vivo or in vitro SSC maturation yielded functional sperm and offspring in at least one animal model (Table 1) (Shinohara et al., 2002; Wu et al., 2012; Sato et al., 2011; Nakai et al., 2010; Liu et al., 2013; Kaneko et al., 2013; Ogawa et
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Induced pluripotent stem cell iPSC technology Jin
2018-11-08
Induced pluripotent stem cell (iPSC) technology (Jin et al., 2012; Singh et al., 2013; Takahashi and Yamanaka, 2006; Tucker et al., 2013a,b, 2014a, 2015) makes it possible to derive cell types in vitro that would otherwise be inaccessible in living patients. This in turn allows one to evaluate the p
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Gene set enrichment analysis GSEA of developmentally
2018-11-08
Gene set enrichment analysis (GSEA) of developmentally regulated genes showed that transcripts from bivalent genes were preferentially upregulated in Dicer-deficient ESCs, comparable with a set of Targetscan-predicted target genes of miR-290–295 (Figure 3B). Eight sets of developmental genes were us
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erk inhibitor br Experimental Procedures br Author
2018-11-08
Experimental Procedures Author Contributions Acknowledgments This work was supported by JST, CREST and MEXT (to E.N.), and MEXT Scholarship Program (to J.L.). Introduction Developmental processes depend on highly orchestrated shifts in the levels of specific mRNAs. While regulation of t
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Our results show that anterior NPCs can give rise
2018-11-07
Our results show that anterior NPCs can give rise to GnRH-expressing ldk378 when cultured with FGF8, which has been shown to promote progenitor cell survival in the anterior neural structures (Forni et al., 2013; Kawauchi et al., 2005). In mouse anterior neural ridge, FGF8 has been shown to positiv
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Go 6976 br Discussion br Experimental Procedures br Author C
2018-11-07
Discussion Experimental Procedures Author Contributions Acknowledgments We thank members of the Azrieli Center for Stem Cells and Genetic Research at the Hebrew University for their contribution, and especially Mordecai Peretz for assistance with the figures, and Ido Sagi for critical re
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The remainder of the paper is organized
2018-11-07
The remainder of the paper is organized as follows. In Section 2, a review of the literature on the topic is presented. In Section 3, our methodology is explained and some data considerations are added. In Section 4, results are displayed and reflections per gender are offered. Section 5 discusses o
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